Acetylated Peptides

Acetylated Peptide Synthesis | N-terminal Acetylated Peptides | Lysine Acetylated Peptides | Post-translational Modified Peptides | Science-Peptide

Need acetylated peptide for epigenetic, signaling pathway or drug discovery? Science-Peptide Biotech provides acetylated peptide synthesis service, N-terminal acetylation, Lys side chain acetylation can be done, 20 years of experience, welcome to contact us.

Why acetylated peptides?
Anyone who has done peptide synthesis knows that acetylating the N-terminus is the most common operation - removing the positive charge, making the peptide more like a natural protein, and blocking some protease attacks.

But acetylated peptides are much more than that. In the cell, acetylation of lysine is a key switch that governs gene transcription, protein stability, and metabolic pathways. To study this, it is not enough to have a naked peptide, but you must have an acetylated peptide with acetylation at a specific location.

 

Who needs it?
Epigenetic: the chromatin state is different depending on which site of the histone is acetylated
In signaling pathways: the binding of proteins may depend on the acetylation of a certain lysine
Screening: Inhibitors targeting the bromodomain, with acetylated peptides as probes
For peptide drugs: N-terminal acetylation can increase the half-life in vivo

1. Type of Acetylation, Depending on What You Need

We have done all these acetylated peptides and have a mature protocol.

 2. acetylated peptides that we can synthesize

• N-terminal acetylation: the last step of solid-phase synthesis is treated with acetic anhydride, which is simple and has high yield.
• Targeted lysine acetylation: direct use of Fmoc-Lys(Ac)-OH, a commercially available monomer, with precise sites and few side reactions.
• Multi-site acetylation: Multiple Lys sites are introduced to acetylate lysine one by one without interfering with each other.
• Acetylation + other modifications: e.g., simultaneous phosphorylation, methylation, or fluorescent labeling to study modification crosstalk.

1. 20 years of experience, all types of acetylation
Acetylation of peptide sounds simple, but it is still a bit tricky to achieve accurate site, complete modification and less impurity. Over the years, we have been involved in many acetylation projects: from the simplest N-terminal acetylation to complex multi-site acetylation of histones, from short peptides to long peptides, we have accumulated experience.

2. Site control: you say acetylate which Lys, is which
Some companies use non-specific methods for lysine acetylation, and the result is that the acetyl groups are scrambled, and you can't tell which site is working. We don't do that:
Fmoc-Lys(Ac)-OH was used to directly sample acetylated lysine as a common amino acid.
Multiple Lys sites were introduced into acetylation one by one using an orthogonal protection strategy without interfering with each other.
Want to leave some of the Lys unmodified? No problem, selective deprotection can be achieved with different protecting groups.

3. Purity on demand, without waste of money
Routine Pure (85-95%):ELISA, pull-down, active primary screening is sufficient.
High purity (95-98%): more stable results in cell and animal experiments.
Ultra-pure (>98%): crystal growth, NMR, purity Raman.

4. Follow-up from initial screening to preclinical
After finding a key acetylated peptide, there are often follow-ups: synthesizing mutants to verify the specificity, scaling up to milligram level for animal experiments, and GMP version for conversion. Science-Peptide Biotech can seamlessly follow up with the same project manager to the end, without repeated communication.

Whether you are doing mechanistic research or drug development, if you need acetylated peptides, you can talk to us.

Give you a detailed quote and feasibility feedback within 24 hours.