Biotinylated Peptides

Biotinylated Peptides | Biotinylated Peptide Synthesis | Peptides for Affinity Purification | Science-Peptide

Need Biotinylated Peptides for affinity purification or ELISA? Science-Peptide provides professional Biotinylated Peptides service, supporting N-terminal, C-terminal, lysine side-chain and other site-specific labeling, high purity, fast delivery, welcome to inquire.

In life science research and diagnostic reagent development, Biotinylated Peptides are ideal for affinity purification, ELISA, flow cytometry, immunohistochemistry, and molecular interactions analysis scenarios due to their ultra-high affinity for Avidin or Streptavidin.Science-Peptide is a leading provider of high quality Biotinylated Peptides. With over 20 years of experience in peptide synthesis, Science-Peptide provides you with high-quality customized Biotinylated Peptides - from the selection of labeling sites, modification types to purity requirements, we tailor-make the design according to your application scenarios to ensure that each Biotinylated Peptide can be used in your experiments. Peptides are customized according to your application to ensure that each Biotinylated Peptide performs stably in your experiments. Whether in milligram quantities for research or gram quantities for diagnostic reagents, we ensure batch-to-batch consistency and activity stability of Biotinylated Peptides.

Biotinylated Peptides are molecules of biotin (Biotin) chemically covalently attached to specific positions in a peptide sequence. Biotin (vitamin B7) is a small molecule (molecular weight 244.31 Da) with a binding constant of up to 10¹⁵ M-¹ to proteins of the affinity family (affinomycin, streptavidin), one of the strongest non-covalent interactions known in nature. This property enables Biotinylated Peptides to efficiently capture target molecules in complex biological systems, or to be used as probes for detection and imaging. In specific experiments, the choice of Biotinylated Peptides often depends on the spatial accessibility of the target molecules and the compatibility of the experimental system.

 

According to the different labeling sites, Biotinylated Peptides can be classified as follows:
N-terminal biotinylation: biotin is attached to the α-amino group of the peptide, and is applied to peptides whose N-terminal group is not involved in functional activity.
C-terminal biotinylation: biotin is attached to the carboxyl terminus of the peptide (usually via a lysine or an additionally introduced cysteine) and is suitable for C-terminal exposed systems.
Side-chain biotinylation: Biotin attached to the ε-amino group of lysine (Lys), the sulfhydryl group of cysteine (Cys), or other non-natural amino acids enables multi-site labeling.

Introduction of spacer arms: Adding spacer arms of different lengths (e.g. 6-aminohexanoic acid, PEG chain) between biotin and peptide can effectively reduce the spatial resistance and improve the binding efficiency, which is especially suitable for affinity-based capture experiments.

1. Multiple labeling sites available
We support the synthesis of Biotinylated Peptides at N-terminal, C-terminal, lysine side chain, cysteine side chain and other sites. For applications where the natural conformation of the peptide needs to be preserved, we will help you choose the most appropriate labeling position to avoid interfering with the functional domain. For example, for enzyme substrate peptides, we usually recommend labeling the biotinylated peptide at the end away from the cleavage site.

2. flexible space arm design
For applications that require a reduction in spatial resistance (e.g. capture experiments on affinity-encapsulated plates), we can introduce spacer arms of different lengths (Ahx, PEG2, PEG4, PEG12, etc.) to allow for more flexible exposure of biotin molecules and to improve binding efficiency. In some demanding SPR experiments, the appropriate spacer arm length is often the key to the success of the experiment, we will recommend the best solution according to your experimental design.

3. High purity and complete characterization
Each Biotinylated Peptide was purified by HPLC and characterized by mass spectrometry (MS) to ensure that the biotinylated peptide was correctly attached and free of residual by-products. For sequences containing multiple reaction sites, we use an orthogonal protection strategy to ensure that the modifications occur at the designated sites. Detailed COA reports are provided upon delivery, including purity, molecular weight, and modification site confirmation. For critical applications, biotin binding activity validation data can also be provided.

4. Flexible in size
From milligram quantities for scientific research to gram quantities for diagnostic reagents, we are able to provide stable production. For projects requiring long-term supply, we can sign batch agreements to ensure batch-to-batch consistency. We have been supplying Biotinylated Peptides to a number of diagnostic reagent companies for several years with a CV of <5% between batches.

5. Compatible with a wide range of modification combinations
Biotin modification can be introduced simultaneously with other modifications (e.g., fluorescent labeling, phosphorylation, glycosylation, PEGylation, etc.) to meet multiple labeling requirements. For example, dual-labeled peptides with both biotin and FITC can be used for simultaneous affinity capture and fluorescence detection, while the combination of biotin and phosphorylation modifications can be used to screen for phosphorylation-specific antibodies.

1. Affinity purification
Biotinylated peptides immobilized on streptavidin resin can be used to purify target proteins or antibodies that bind specifically to them. This method is gentle and efficient, and is widely used in protein interaction studies. We have synthesized Biotinylated Peptides for a customer to purify their newly discovered transcription factor, and the purification rate was increased by 30% in a single run.

2. ELISA with microplate assay
Biotinylated Peptides can be used as encapsulated antigens or capture probes for colorimetric detection in conjunction with enzyme-labeled Streptavidin (SA-HRP) for antibody screening, epitope identification, or diagnosis of autoimmune diseases. A variety of spacer arm options are available to optimize assay sensitivity.

3. Flow cytometry and cell sorting
By labeling cell surface receptors with Biotinylated Peptides and adding fluorescently labeled streptavidin, qualitative and quantitative analysis of receptors or magnetic cell sorting can be achieved. For such applications, we recommend the use of Biotinylated Peptides with PEG spacer arms to minimize spatial resistance.

4. Surface plasmon resonance (SPR) and biofilm interference (BLI)
Biotinylated Peptides immobilized on Streptavidin sensor chips can be used to analyze the binding kinetics of peptides to target proteins in real time, which is a common method for drug screening. We have provided high quality Biotinylated Peptides for SPR analysis to many pharmaceutical companies with excellent data reproducibility.

5. Immunohistochemistry and tissue staining
Biotinylated Peptides can be used as probes to detect specific targets in tissue sections and amplify the signal by combining them with the enzyme labeled streptavidin. For complex samples such as paraffin sections, we offer Biotinylated Peptides with a long spacer arm for improved staining.

6. Pull-down coupled with mass spectrometry
Biotinylated Peptides can be used to "pull down" interacting proteins from cell lysates and identify new binding partners by mass spectrometry. Biotinylated peptides are available in a biotin labeling option that does not interfere with the mass spectrometry signal.

1. Extensive experience in grooming
We have synthesized tens of thousands of biotinylated peptides, covering a wide range of labeling sites, spacer arm lengths and modification combinations. Whether it is a simple labeling or a complex combination (e.g., both biotin and fluorescein), we can achieve it with precision. Customers have reported that our Biotinylated Peptides have low background and strong signal in ELISA and SPR.

2. Rigorous quality control
In addition to routine HPLC and MS, for critical applications we can provide biotinylated activity validation to ensure the reliability of each batch of Biotinylated Peptides in real-world experiments. We also support NGS validation for library products, but for single peptides, QC is sufficient.

3. Flexible production scale
We have a stable production system from research grade milligrams to diagnostic reagent grade grams, and can provide multi-batch consistency testing to meet long-term research or product development needs. For GMP grade Biotinylated Peptides, we also have a mature production system.

4. Rapid delivery
Routine Biotinylated Peptides orders (length <30 aa, standard modifications) can be completed within 10-15 business days. Expedited service can be negotiated to 5-7 business days.

5. Professional technical support
Our project managers have a background in peptide chemistry and can provide you with advice on labeling site selection, spacer arm design and application protocols. Many of our customers have commented that our advice is very practical after the first consultation and has helped them avoid many common pitfalls.

A diagnostic reagent company needs a line of Biotinylated Peptides for autoimmune disease detection, N-terminal biotinylated, with PEG4 spacer arm, purity >95%. We successfully delivered 5 grams of the product, which was validated by ELISA with good activity, and is now used in their commercialization kits. Subsequently, they have added several batches of the product, all of which are of the same standard.

Laboratory of Immunology in Higher Education: The study of T cell epitope recognition requires multiple overlapping peptides biotinylated at different sites. We synthesized 30 biotinylated peptides, which were labeled at N-terminal or C-terminal, and used them in the preparation of MHC tetramers, which helped them to identify new epitopes successfully, and the related results have been published in the Journal of Immunology.

A multinational pharmaceutical company needed to screen phage display libraries for antibody drug discovery and commissioned us to synthesize biotinylated target protein mimetic peptides. We designed and synthesized a biotin-containing cyclic peptide library with a library capacity of 10⁸, which was successfully used in their screening program, and several lead compounds have been obtained so far.

Whether you need a simple N-terminal Biotinylated Peptide for an ELISA or a complex multisite labeled peptide for a multiplex assay, Science-Peptide has the expertise and rigor to serve you. Our team is happy to discuss with you in depth before the start of your project to ensure that the design perfectly matches your experimental needs.