Neoantigen Peptides for Cancer Immunotherapy

Neoantigen Peptides | Personalized Neoantigen Peptide Synthesis | Long/Insoluble Peptide Customization | Science-Peptide

Need high quality Neoantigen Peptides for personalized cancer vaccine or T-cell screening? Science-Peptide provides Neoantigen Peptide synthesis service, supporting long peptides, refractory sequences, multi-site modification, high purity, fast delivery, to help tumor immunotherapy research. Welcome to contact us.

As anyone in tumor immunotherapy knows, finding a good target is not easy, and synthesizing the predicted Neoantigen Peptides with high quality is also a challenge. These peptides are often long, insoluble, and contain hydrophobic amino acids, which are impossible to be synthesized by normal synthesis methods. We have been in the peptide field for 20 years, and we have received many orders for Neoantigen Peptides, and we have encountered all kinds of difficulties - some need to be delivered in time for the patient's therapeutic window, some peptides can't be stirred up well in water, and some have several mutation sites crowded together. It was these experiences that led us to build a synthesis system specifically for Neoantigen Peptides, and every step, from route design to purification strategy, was aimed at solving problems.

Neoantigen peptides are simply unique protein fragments produced by mutations in cancer cells. These mutations include point mutations, insertion deletions, and gene fusions that result in amino acid sequences that are different from those of normal cells. When these peptides are presented to the cell surface by MHC molecules, the immune system recognizes that "this is bad" and attacks.

In practice, Neoantigen Peptides fall into two main categories:

Long peptides (25-35 aa): encapsulate the mutation site and surrounding sequences, need to be swallowed by dendritic cells, processed and then presented, commonly used in personalized vaccines.

Short peptides (8-11 aa): predicted or validated MHC binding epitopes that can be used to stimulate T cells directly or for tetramerization.

Regardless of whether it is a long peptide or a short peptide, Neoantigen Peptide synthesizers have the same headaches: the sequence is hydrophobic, it is easy to be "struck" by the synthesizer, the yield is low during purification, and it has to be delivered in time for the patient's treatment.

1. Specializing in long and insoluble peptides
A large proportion of the Neoantigen Peptides that come to us have more than 25 amino acids. Conventional methods are often ineffective in dealing with these peptides. Over the years, we've figured out a few ways to deal with them:

• Fragmentation: The long peptide is cut into several segments and synthesized separately, each segment is purified first, and then assembled on resin or in liquid phase. In this way, the quality of each segment is guaranteed, and the yield and purity of the final product are higher.
• Microwave-assisted synthesis: Some amino acids are reluctant to react with microwave heating, the reaction efficiency will be increased immediately, and there are fewer by-products.
• Special solvent purification: some peptides are not soluble in common solvents, so we add DMSO, guanidine hydrochloride and other strong solvents during purification to wash them off from the column, and they can still maintain good properties after lyophilization.

2. Not a single mutation site is missing
The essence of Neoantigen Peptides lies in those few mutation sites, which can never be mistaken when synthesizing. We use Fmoc solid-phase synthesis to introduce non-natural amino acids directly, and we keep an eye on the data after each step of coupling to make sure that the amino acid sequences of the mutation sites are exactly the same as the design.

3. Speed is hard
The therapeutic window for tumor patients is so wide that peptides are meaningless if they are late. We've given Neoantigen Peptides the green light:

• Short peptides (<20 aa): 10 working days
• Long peptides (20-35 aa): 12-18 working days
• Expedited: 7 business days is negotiable if you are in a real hurry

Last year, there was a customer who had already organized his patients and was waiting for the peptides to make a vaccine. Several of our synthesizers worked in shifts to deliver all 30 peptides to him within two weeks.

4. Strict purity and endotoxin criteria
When you use something on people, you can't afford to compromise on quality. We offer Neoantigen Peptides:

• Purity:>90%, >95%, >98% Whatever you choose
• Endotoxin:<2 EU/mg, or customized according to customer requirements
• Microbial limit: in accordance with the Pharmacopoeia
• Residual solvents: in accordance with ICH Q3C

Each peptide's COA report is accompanied by HPLC and MS graphs, and the data can be traced, so many customers directly take it and put it into the declaration.

5. Grooming can also be done
Some experiments require a "handle" for Neoantigen Peptides:

• Biotin labeling: for MHC tetramer
• Fluorescent labeling (FITC, TAMRA, Cy5): for flow-through staining
• Carrier protein coupling (KLH, BSA): for immunization of animals
• Palmitic acid modification: making vaccines better at finding lymph nodes

We can do it all together, without you having to find two suppliers.

1. 20 years of experience and many difficult problems
We have been in this field for 20 years, and we have encountered all kinds of difficult peptides, and we have a way to deal with long chains, hydrophobic, and repetitive sequences that are common to Neoantigen Peptides, and we will not easily tell you that we can't do it. Some peptides that others think cannot be made, we can often find a way out after a few days of research.

2. Keeping pace with the pace of treatment
Tumor treatment can't wait. We fast-tracked the neoantigen peptide program, prioritizing the entire process from evaluation to delivery. Many customers later told us that they chose us because we delivered the peptide to them in the time they needed.

3. Quality can be verified
Whether it is crude peptide for scientific research or high purity low endotoxin peptide for preclinical research, we treat them all the same: not less than one of the quality inspection and not less than one of the data that should be given. Many customers directly copy our COA report and put it into the declaration documents.

4. From milligrams to grams, without replacement
Early detection is only a few milligrams, toxicology studies are tens of grams, clinical trials are hundreds of grams - from start to finish, we can take it all. You don't have to change the same species, and you don't have to worry about it.

5. Your sequence, as only you and I know it
The new antigen sequence is the core asset of each client. We sign a confidentiality agreement, project information is only known to a few people in the project team, and the data system is secure. In all these years, there has never been any leakage.

A cell therapy company: they have a patient with advanced melanoma who is waiting for a personalized vaccine. 30 long peptides, 25-35 amino acids, 8 of which are extremely hydrophobic, and the yield of purification by ordinary methods is extremely low. We used fragment condensation and special solvents to purify the peptides, and delivered all of them to the customer within two weeks, and the endotoxin was controlled to be below 0.05 EU/mg. Later, after the patient's treatment, several new antigen-specific clones were amplified by T-cell assay, and the customer specifically called us to say that "the quality of the peptide helped a lot".

A top oncology institute: they want to screen a batch of neoantigen-reactive TCRs and need 200 predicted peptides (9-11 aa). We synthesized all the peptides in three weeks using a high-throughput platform, and each peptide was >95% pure and confirmed by MS. The customer used these peptides to make tetramers and screened out several high-affinity TCRs, which are now in preclinical development.

Whether you're just starting out in preclinical research or you're already in a clinical trial and need a personalized peptide for a vaccine, Science-Peptide can handle it. Our technical team is ready to listen to your needs.