Overlapping peptide library

  Overlapping Peptide Library Synthesis | Overlapping Peptide Library | Epitope Mapping Peptide Library | T-Cell Screening Peptide Library | Science-Peptide

Need Overlapping peptide library for epitope mapping or T-cell screening? Science-Peptide provides professional Overlapping peptide library synthesis service, covering the whole protein sequence, with flexible peptide length and overlap, and fast delivery in 2-3 weeks. Welcome to contact us.

Overlapping peptide library?
Anyone who does immunological research knows that the most direct way to find out where an antibody binds to a protein, or which section a T cell recognizes, is to cut the protein into small pieces and try them one by one.

Overlapping peptide library is such a small set of pieces - based on the sequence of the target protein, a series of short overlapping peptides are designed to cover the entire protein. Each peptide shares an overlapping region with its neighboring peptides, ensuring that no possible epitope is missed.

 

An example:A protein of 300 amino acids, designed as a 15 amino acid peptide, overlapping 11 amino acids, then the first peptide is 1-15, the second is 5-19, and the third is 9-23 covering all the way to the end.

 

This kind of design is common in immunology: th:

• Antibody epitope mapping: find out where antibodies actually bind
• T-cell epitope screening: to find out which fragments are recognized by T cells
• Vaccine development: screening for candidate epitopes that induce immunoprotection
• Autoimmune disease research: identifying targets that attack themselves

1. Peptide library design: help you calculate the parameters
Anyone who has done overlapping peptide library knows that the choice of peptide length and overlapping degree directly affects the experimental results. If the peptide is too short, the conformational epitope may be missed; if it is too long, the synthesis will be difficult and costly. We will give you a solid suggestion according to your research purpose:

We can handle special situations:

• Cysteine-rich regions: easily form disulfide bonds, can be replaced with Ser or Ala
• Where hydrophobicity is high: poor solubility, add some labeling or shorten peptide length
• Known non-functional areas: skip them, save money and time

2. Synthetic Capacity: from a Few Dozen to a Few Hundred

3. Quality control: every peptide is documented
For every peptide in the Overlapping peptide library, we give:

• MS mass spectrometry: molecular weights at a glance
• HPLC purity: crude or purified, data speak for themselves
• COA report: one peptide at a time, searchable by pore position or sequence

Want to do more? Yes:

• Peptide Content Measurement: If you want to quantify accurately, do this
• Solubility Test: Tells you what solvent to use to dissolve
• Amino acid composition analysis: to confirm the ratio of each amino acid

1. We've been doing this for 20 years
Overlapping peptide library sounds like "one batch of peptide", but there are still a lot of details to achieve stable batch, complete data and on-time delivery. Over the years, we have done hundreds of Overlapping peptide library projects for our customers, ranging from dozens of peptides to hundreds of peptides, and the process has been running smoothly for a long time.

2. High flux, also fast
For a standard library of 96 peptides, it takes 2-3 weeks, and for a library of about 200 peptides, 3-4 weeks. If the project is urgent, it can be divided into batches - half of the library can be taken first to start the experiment, and the rest can be added one after another, without waiting.

3. Purity on demand, without waste of money
Crude (70-85%): for initial screening, see which bars have signals first, save money.
Routine pure (85-95%): most cell experiments, cost-effective enough.
High purity (95-98%): For quantitative or in vivo experiments, the results are more stable.

4. Complete data, no worries about publication
The MS and HPLC data of each peptide will be given to you, organized in Excel, so that you will know exactly which peptide corresponds to which well, what is the purity, and whether the molecular weight is correct or not. When you write an article, you can put it into the Supplementary Information, so you don't need to organize it by yourself.

5. Need a modifier? You can add that, too
Some experiments require labeled peptides:
Biotin labeling: do streptavidin capture or detection
FITC labeling: for fluorescence detection
Phosphorylation modifications: a study of phosphorylation-dependent recognition that
When you synthesize it, you can add it in directly, so you don't have to come back and do it again.

Form of delivery:
Individual centrifuge tube dispensing
Accompanying documents:
Excel summary table (sequence, MW, purity, MS file links for all peptides)
Independent COA for each peptide (PDF)
library design summary report
Optional: solubility data, peptide content, amino acid composition analysis
Before each Overlapping peptide library is sent out, we check to make sure that the number of peptides is correct and the data is complete.

Whether for epitope mapping, T-cell screening, or vaccine development, we can help you design and synthesize high-quality Overlapping peptide libraries.

I need you to tell me:
Protein sequence (or UniProt ID)
Peptide length and overlap (we can help you design this)
Number of peptides (approximate range)
Purity requirements
Need for modification (biotin, FITC, etc.)
How much to ask for (milligrams per peptide)

Give you a quote and delivery plan within 24 hours.