PEGylation of Peptides

PEGylation of Peptides | PEG-Modified Peptides | Polyethylene Glycolated Peptides | PEG2/4/8/2000/5000 | Science-Peptide

Need PEGylation of Peptides to extend half-life or improve solubility? Science-Peptide provides professional PEGylation of Peptides synthesis service, from short chain PEG2/PEG4/PEG8 to linear PEG2000/PEG5000, N-terminal/Cys/Lys targeted modification. 20 years experience, high purity delivery. Welcome to inquire.

Why PEGylation of Peptides?
Anyone who has worked with peptide drugs has experienced that a good peptide has good activity in vitro, but as soon as it enters the body, it's gone - it's filtered too quickly by the kidneys, or it's been cleaved by proteases.

 

PEGylation of Peptides is the solution to this problem. By attaching a polyethylene glycol (PEG) chain to the peptide, the molecular weight becomes larger and glomerular filtration slows down; the PEG chain also blocks protease attack and reduces degradation. The result is a longer half-life and less frequent administration.

 

But PEG is more than just a tube to extend time. Short-chain PEGs (e.g. PEG2, PEG4, PEG8) are often used as linkers or to slightly improve solubility without changing the overall molecular weight too much. The choice of PEG depends on the effect you want.

 

What conditions are suitable for PEGylation?
Need linker molecule: coupled fluorescent, biotin or toxin → choose short chain PEG (PEG2/4/8)
Poor solubility: PEG is hydrophilic and improves solubility → short-chain or medium PEG is fine
Need for slow release: extended half-life of macromolecular PEG → 20k linear PEG

1. PEG Type, from Shortest to Longest

2. The Connection, Depending on Where You're Connected

1. We've been doing this for 20 years
PEGylation of Peptides sounds like "linking PEG chains", but there are many kinds of PEGs, and the reaction efficiency and purification difficulty are different from those of ordinary peptides. Over the years, we have helped our customers with a variety of PEGylation projects: from the shortest PEG2 linker to 5kDa linear PEG long-lasting modification; from small peptides to long peptides; from single modification to multi-modification, all of them have accumulated experience.

2. Flexible connectivity, wherever you say
Some companies only do N-terminal PEGylation, so they can't change the site. We are different:
N-terminal PEGylation: conventional operation, good selectivity
Cys spot PEGylation: if free Cys is present, use maleimide, high specificity
Lys Fixed Point PEGization: If there is only one Lys, or if you want multiple points, you can control that
Short-chain PEG ligation: particularly suitable as a linker arm for ADC, PROTAC or peptide-coupled drugs

3. A full range of PEGs, from the shortest to the longest, is available
Short chain monodisperse PEG: PEG2, PEG4, PEG8, PEG12 whichever size you need
Linear PEG: 2k, 5k, 10k, 20k
Mono-dispersed PEG: uniform molecular weight, suitable for pharmaceuticals

4. Purity on demand, without waste of money
Conventional pure (85-95%): sufficient for primary screening for activity, PK studies
High purity (95-98%): animal experiments, efficacy studies, more stable results

5. Follow all the way from screening to amplification
After finding a candidate with preserved activity and prolonged half-life after PEGylation, it is often necessary to amplify it and make a GMP version, Science-Peptide can work seamlessly with the same project manager to the end, without repeated communication.

Whether you want to extend your half-life, improve solubility, or make a linker, talk to us.
Give you a detailed quote and feasibility feedback within 24 hours.