Truncation Peptide library

Truncation Peptide library | Truncation Peptide library Synthesis | Minimal Active Fragment Screening | Peptide Truncation Library | Science-Peptide

Need to find out the smallest active fragment of a peptide? Science-Peptide provides professional Truncation Peptide library synthesis service, with stepwise truncation from the N-terminal, C-terminal, or both ends, rapid delivery, and help you pinpoint the functional core region. Welcome to contact us.

What is the Truncation Peptide library?
You have a peptide on hand that has good activity, but is too long - expensive to synthesize and not yet stable. You want a shorter version, but don't know where to start cutting.

 

This is what the Truncation Peptide Library does. It cuts off amino acids from the N-terminal, C-terminal, or both ends of the parent peptide step by step, and cuts out a series of shorter and shorter peptides, and then measures the activity of each one of them until it finds the smallest active fragment that is "no shorter than the shortest one".

 

This works particularly well in the following scenarios:

• Minimum Active Fragment Screening: Identify the shortest sequence necessary to maintain activity
• Drug optimization: shorten peptide chain, reduce cost, improve stability
• Constituent relationship studies: looking at which amino acids are dispensable at both ends
• Epitope fine-tuning: identification of core regions for antigen recognition

1. Multiple Truncation Methods, as Required

An example is a 10 amino acid peptide with the sequence YGRKKRRQRRR.
N-terminal truncated banks: 1st cut off Y (GRKKRRQRRR), 2nd cut off G (RKKRRQRRR), and so on
C-terminal truncated bank: 1st cut off R (YGRKKRRQRR), 2nd cut off R (YGRKKRRQR), and so on

2. Pool Design: Step-By-Step Truncation, Covering the Entire Length

3. Synthetic Capabilities

4. Quality control: data for each peptide
For each peptide in the Truncation Peptide Library, we offer:
MS Mass Spectrometry: Confirmation of correct molecular weight (any change in molecular weight after truncation counts as good)
HPLC purity: crude or purified purity data
COA report: one per peptide, searchable by length or truncation

Optional services:
Peptide Content Measurement: If you need accurate quantification, do this
Solubility test: Recommendation of suitable solvents
Amino acid composition analysis: Confirmation of correct sequence

1. We've been doing this for 20 years
Truncation Peptide library sounds like "a batch of shorter and shorter peptides", but there are a lot of details to make sure that each peptide is correct, the purity is consistent, and the delivery is on time. Over the years, we have helped our customers to make various kinds of truncation libraries, ranging from 10 peptides to 100 peptides, and the process has been smooth for a long time.

2. The cut-offs are flexible and can be done in any manner
Some companies only do single-ended cutoffs, and they're blind to any other way of doing it. We're different:
N-terminal truncation: routine operation
C-terminal truncation: as skillful as
Both ends are simultaneously truncated short:you can design combinatorial libraries that
Unequal step truncation: a large step of coarse screening followed by a fine step of precise localization

3. Purity on demand, without waste of money
Crude (70-85%): for initial sieving, to see which lengths are still active and to save money.
Regular pure (85-95%): most of the activity experiments, cost-effective enough.
High purity (95-98%): Needed for precise quantification or structural studies, more stable results.

4. Data integrity and credibility of results
The MS and HPLC data of each peptide will be given to you and organized in Excel, which length corresponds to which peptide, what is the purity, and whether the molecular weight is correct or not, all of which are clear. You can use them directly when you write articles.

5. Need a modifier? You can add that, too
Some experiments require labeled peptides for detection:
Biotin labeling: to facilitate capture or detection
FITC labeling: for fluorescence detection
Phosphorylation modification: study of phosphorylation-dependent activity of
When you synthesize it, you can add it directly, so you don't have to come back and do it again.

6. Follow through from screening to validation
After finding the smallest active fragment, it is often necessary to do more follow-up work, such as optimizing the sequence, scaling up for animal experiments, and developing the process, Science-Peptide can seamlessly connect these stages - the same project, the same counterpart, no need to re-adjust.

Form of delivery:
96-well plate (one peptide per well, lyophilized powder, labeled with truncation method and length)
or individual centrifuge tube dispensing (on demand)
Labeling information: truncation mode, length, sequence, molecular weight, purity

Accompanying documents:
Excel summary table (sequence, MW, purity, MS file links for all peptides)
Independent COA for each peptide (PDF)
Library design summary report (describing how each peptide corresponds to a truncation and its length)
Optional: solubility data, peptide content determination
Before each Truncation Peptide Library is sent out, we check to make sure that the truncation method is correct, the number of peptides is correct, and the data is complete.

Whether you are looking for the smallest active fragment of an antimicrobial peptide or pinpointing an antibody epitope, we can help you design and synthesize high-quality Truncation Peptide libraries.

I need you to tell me:
parent peptide sequence
Truncation method (N-terminal, C-terminal or both)
Truncation step (1 or more cuts at a time)
minimum retention length
Do you need to add the parent peptide control
How much of each peptide to
purity requirements
Need for modification (biotin, FITC, etc.)

When do you want it?
Give you a quote and delivery plan within 24 hours.