Custom Peptide Modification | Peptide Chemical Modification| Fluorescent-labeled Peptide,Phosphorylated Peptide,Stapled Peptide | Science-Peptide
Meta Description
Need peptides with special modifications?Science-PeptideCustom Peptide Modification service covers more than 200 types of modifications, such as fluorescently labeled, phosphorylated, glycosylated, PEGylated, stapled peptides, etc. With 20 years of experience, we are able to provide stable delivery of complex modifications. Please feel free to contact us.
Need a peptide with modification for your research? Let us do it.
Why do you need Custom Peptide Modification?
When doing research, we often need to add some "special functions" to peptides - such as attaching a fluorescent group to see how it runs in the cell, or adding a phosphoryl group to mimic the activation state of proteins, or making a stapled peptide to make the structure more stable and not easily degraded by enzymes. degraded by enzymes.
These modifications don't sound difficult, but when it comes to synthesizing them, the question arises: are the modification sites correct? Is the yield enough? Is the purity good enough? Will multiple modification combinations fight?
Science-Peptide's Custom Peptide Modification service is specialized in solving these problems, with 20 years of experience in peptide modification and more than 200 types of modifications, we can find reliable synthetic routes for simple biotin labeling or complex multi-modification combinations.
Below are some of the modification types we support (partial list):
|
Types of Modifications |
Common Modifications |
Application Scenarios |
|
Fluorescent Labeling |
FITC (Fluorescein Isothiocyanate), FAM (Fluorescein Amidite), TAMRA (Tetramethylrhodamine), Cy3/Cy5, Rhodamine, Alexa Fluor series |
Cell imaging, flow cytometry, fluorescence polarization |
|
Affinity Tagging |
Biotin, His-tag (Histidine tag), Flag-tag, HA-tag (Hemagglutinin tag) |
Affinity purification, pull-down assays, and SPR (Surface Plasmon Resonance) analysis |
|
Stability Modifications |
PEGylation (modification with Polyethylene Glycol), fatty acid modification (e.g., palmitic acid, myristic acid), cholesterol modification |
Extension of half-life, improved solubility, enhanced membrane permeability |
|
Post-Translational Modification Mimicry (PTM mimicry) |
Phosphorylation (pSer: phosphoserine / pThr: phosphothreonine / pTyr: phosphotyrosine), glycosylation, acetylation, methylation |
Signal pathway research, enzyme substrate identification, protein-protein interaction |
|
Structural Modifications |
Amidation, cyclization, stapled peptide (a stabilized peptide by covalent linking) |
Stabilized conformation, increased resistance to proteolysis, enhanced activity |
|
Incorporation of Unnatural Amino Acids |
D-amino acids, non-natural amino acids, isotopic labeling (¹⁵N: nitrogen-15 / ¹³C: carbon-13) |
Structure–activity relationship (SAR) studies, NMR (Nuclear Magnetic Resonance) structural analysis, metabolic stability testing |
|
Photosensitive Probes |
Photo-crosslinking group (Bpa: p-Benzoyl-L-phenylalanine), photolabile protecting group (Nvoc: Nitroveratryloxycarbonyl) |
Photoaffinity labeling, photo-controlled release, spatiotemporal specificity studies |
|
Precursors for Radiolabeling |
DOTA (1,4,7,10-Tetraazacyclododecane-1,4,7,10-tetraacetic acid), NOTA (1,4,7-Triazacyclononane-1,4,7-triacetic acid) for metal chelation |
PET/SPECT imaging (Positron Emission Tomography / Single Photon Emission Computed Tomography), radiotherapeutic drug development |
Why choose us for Custom Peptide Modification?
1. More than 200 kinds of modifications, we have basically everything you can think of.
Customers often come to us with literature and ask: "Can you do this modification?" We take a look at it, and most of the time the answer is: "We have done it, and we can do it better." Because over the past 20 years, we've accumulated enough experience and failures with trims to know what works and what doesn't.
2. Complex modification combinations? We've disassembled a lot of them.
Some peptides need to carry several modifications at the same time: N-terminal fluorescent labeling, intermediate phosphorylation, C-terminal amidation. This kind of combination is very demanding on the synthesis strategy, and one wrong step will lead to a total failure. We optimize the amino acid protection strategy and coupling conditions to ensure that each modification is in the right place and does not interfere with each other.
A true story: a customer wanted to synthesize a 25 peptide with 3 modifications (phosphorylation + biotin + fluorescent labeling). After asking several companies, they either said they couldn't do it or said it would take at least 6 months. After evaluation, we redesigned the synthesis route and delivered the product in 6 weeks, with a purity of more than 92%, and the customer even mentioned us in the acknowledgement when he sent the article later.
3. Difficult modifications that we are better at instead
Multiple disulfide bonds: up to 4 pairs to ensure the correct orientation.
Stapled peptide: all hydrocarbon chain stapled, improve helical stability and cell penetration.
Glycosylation: O-conjugation, N-conjugation, complex sugar chains can also be on.
Hydrophobic long peptide: hydrophobic peptide itself, more difficult to solubilize with modification? We have a special solvent system that takes care of that.
4. Quality control is not a formality, each peptide has an "ID card".
The quality control of modified peptides is more troublesome than that of ordinary peptides - not only do we need to check whether the molecular weight is correct or not, but we also need to confirm whether the modification site is the specified one. We routinely provide:
MS/MS secondary mass spectrometry: direct proof that the modification is attached to the position you want.
HPLC purity analysis: the purity is what it is, no falsehoods.
Optional tests: peptide content, endotoxin, moisture, amino acid composition analysis, add as needed.
5. From milligrams to kilograms, from R&D to production
A modified peptide performs well in the screening stage, and then you want to enlarge it for animal experiments or even go clinical? No problem. We can complete the connection from small amount to cGMP production on the same platform, and the modification method of the amplification process remains the same, which saves the trouble of process transfer in the later stage.
What Can Custom Peptide Modification Help You Achieve?
|
Research Directions |
Recommended Types of Modifications |
Value of Modifications |
|
Cell Imaging and Tracing |
FITC (Fluorescein Isothiocyanate), TAMRA (Tetramethylrhodamine), Cy5, biotin |
Visualization and localization, tracking of intracellular peptide distribution |
|
Signal Transduction Studies |
Phosphorylation (phosphorylation), acetylation (acetylation), methylation (methylation) |
Mimicking post-translational states to study the functional impact of modifications |
|
Drug Screening |
Stapled peptide, PEGylation, fatty acid modification |
Improved stability, permeability, and activity |
|
Enzymatic Analysis |
Fluorescence quenching pair (FRET peptide: Förster Resonance Energy Transfer peptide), phosphorylated peptide |
Real-time monitoring of enzyme activity, screening of inhibitors |
|
Vaccine Development |
Glycosylation (glycosylation), lipidation (lipid modification) |
Enhanced immunogenicity, mimicking native antigens |
|
Structural Biology |
Isotopic labeling (¹⁵N: nitrogen-15, ¹³C: carbon-13), selenomethionine (a selenium-substituted methionine) |
NMR (Nuclear Magnetic Resonance) and X-ray crystallography studies |
|
Diagnostic Reagents |
Biotin, fluorescent labeling, DOTA (1,4,7,10-Tetraazacyclododecane-1,4,7,10-tetraacetic acid) |
Capture probes, detection probes, imaging probes |
Delivery and Quality Control
Product form: lyophilized powder, centrifuge tubes or syringes.
Documentation: COA report (with HPLC profile and MS profile), synthesis report (optional).
Additional tests: peptide content, moisture, endotoxin, microbiology, amino acid composition, sequence validation - on demand.
We understand that the quality of a modified peptide directly determines the success or failure of your subsequent experiments. Therefore, before each peptide is shipped out, we will check it repeatedly to ensure that the modification site is correct, the purity is up to standard, and the data can be traced.
Three real examples, see how they use it
Case 1: Demand from a top cancer center
They want a p53-derived peptide with dual modification (fluorescent labeling + phosphorylation) to study protein interactions. The problem is that the peptide itself is hydrophobic and almost insoluble after adding the two modifications. We figured out a way to introduce a temporary solubilization tag, and then remove it after synthesis, and finally got the target peptide. The client used it to make key data, and the article was published in Cancer Cell.
Case 2: European biotech company
They are developing peptide-coupled drugs and need to attach toxic molecules at specific positions. We synthesized the modified peptide with orthogonal protecting groups first, and the client did the subsequent attachment by themselves. Because we control the modification site accurately, they have good selectivity in the linkage and the yield is higher than expected.
Case 3: Domestic research team
They need a batch of stapled peptides for antibacterial activity screening. The stapled peptides were difficult to synthesize and the yield was generally low. We optimized the introduction of olefinic amino acid and ring-closing conditions, and finally delivered a stapled peptide with purity >90% and yield 30% higher than they expected, and the activity screening was successfully completed.
More than just modified peptides, we can do much more
Getting a high-quality modified peptide is often just the first step in research. There may be more to follow:
Validate activity
Scale up the synthesis for animal studies
Develop process for clinical studies
cGMP production for clinical trials
Science-Peptide can be with you all the way. We don't just sell you a peptide, we are your technology partner from discovery to development. When you choose our Custom Peptide Modification, you have a team behind you that understands chemistry as well as biology.
Talk about your needs?
Tell us your sequence information (or target background), the type of modification you need, quantity and purity requirements. within 24 hours you will receive professional feasibility feedback and a quote.
